I am developing model for my preliminary studies to check my compound's antidiabetic activity. For this, I tried two different doses i.e. 50 mg/kg and 60 mg/kg of STZ two different times and checked FBG 72 hours later. With the first dose I found no elevated glucose level while with the second dose, my rats died after one day. I need suggestions for developing this model.
Note; as this is just a trial that's why I did not go for a high-fat diet. Please help me in this regards.
@ Anum Mushtaq you can get help from an expert in injecting IP INJECTION.
2nd, after administration, give sucrose 20% for 24 hours to minimize the death of the animal.3rd, stz (should use good reagent e.g. sigma) should be handled maintaining the environment room temperature deduced it activity 4th, you may apply a dose at 40mg/kg. 5th, the age and species of animal should be revised before performing.
you may use aklternative procol also.
Article Study of Hypoglycemic Properties of Aqueous Fresh Leaves Ext...
You can check out Alloxan induced diabetic model, or HFD with low stz diabetic model
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