Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

No title

Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

What is the experimental set up/or protocol to measure distribution of the light emitted?

Hello, Could anyone here please tell me the experimental set up, or the protocol to be able to measure the angular distribution of the light emitted (fD) by some OLED device? I have seen that...

10 February 2020 7,932 2 View

How to measure HRV-PRV agreement using Bland-Altman plots from Excel (step-by-step guide)?

Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...

25 November 2019 3,736 3 View

What is the minimum number of PP intervals required for accurate and reliable extraction of Pulse Rate Variability (PRV) from PPG signals?

The minimum number of RR intervals required for reproduce several metrics derived from HRV (e.g., HF power) can be found in the literature (Richards et al., 2010). However, what about PPG signals...

02 June 2019 353 2 View

Any fast publishing Scopus indexed journal ?

Who can help with a link to the a fast publishing scopus indexes journal. I am looking at a journal that will respond in like 3-5 weeks

19 August 2018 1,327 18 View

Metagenomics databases. Which one choose?

In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...

11 January 2018 858 3 View

Meshing Advise for 2 phase flow ANSYS FLUENT simulation?

I am having difficulty meshing the attached geometry for a two phase mixing type application where I care about the volume fraction at various points along the tubes. I have defined 3 bodies Body...

05 December 2017 1,306 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

How to test the equality of two proportions with multiple samplings?

02 February 2021 9,574 5 View

How can I implement a Generalized Estimating Equation method on spatially autocorrelated time-series data?

I used Generalized Estimating Equations (geepack in R) on my regularly monitored (twice a year) plant abundance data in grid cells to evaluate their trends of density. Since these are time-series...

26 January 2021 3,833 1 View

Why is there no ANOVA output for a certain predictor variable in my GLM (in R)?

Configurations: Rstudio v1.3.1093 Packages: car, lme4, MuMIn, DescTools Model: myGLM

22 January 2021 5,284 6 View

How to confirm my participation and authorship of the article?

Dear staff, I was one of the author's group of two articles - " Toxicity Related to Misuse (Non-Medical Use) of Tramadol: Experience of the European Drug Emergencies Network Plus (Euro-DEN Plus)...

18 January 2021 2,351 2 View

Can use the same vector for overexpression and transient expression?

Dear Researchers, I am using pCAMBIA1301/1302 vectors for overexpression study, so can use the same vectors for transient expression? or need to design another vector for transient expression;...

08 January 2021 7,975 2 View

How to calculate age-standardized prevalence ratio with 95%CI?

I need to calculate the prevalence ratios to show the trend of prevalence of a drug group used by pregnant women over the years (2001-2018). I would like to use the year 2001 with age distribution...

05 January 2021 7,986 5 View

What is the right statistical test?

I have conducted a study in which the participants are divided in two separate groups according to the language of instruction. Also, they are divided in three different groups according to age....

04 January 2021 1,361 17 View

Please How can I compute confusion matrix for Bias reduction generalized linear model (brglm) in R?

I was fitting binary logistics regression with glm function and I discovered the standard error was extremely high, hence, I tried brglm and the standard error is now okay. So, I have issue...

20 December 2020 3,807 4 View