Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

Does anyone has good references to "Organic x Inorganic CO2 in the atmosphere"? Volcanos x Burning fossil fuels?

I need more details and references about: the increase in carbon observed in the atmosphere is the organic origin CO2, not the inorganic origin CO2 released when a volcano erupts. Evaluation of...

19 March 2020 948 3 View

Would be Principal component analysis - PCA the best way to analyze GC-MS data?

I am working with a population of 120 plants which I collected fruit samples from each plant to run in GC-MS. Currently, I have a big dataset with over 1000 volatile compound. What would be the...

04 April 2019 8,800 3 View

Dear colleagues, please lmn what volume do you use for intranasal instillation in rats?

I am in doubt as to the best volume for intranasal instillation in rats, what volume ensures the best distribution of the particles?

20 February 2019 2,920 1 View

Techniques for projecting a 2x1 patch antenna array?

I would like to project a patch antenna array consisting on two rectangular patches for resonating in two distinct frequencies. Are there any good references to learn how to correctly understand...

06 January 2019 9,686 3 View

Does anyone have a protocol to perfom complex-DNA interaction assay via UV spectroscopy?

22 November 2018 1,513 3 View

Does anyone have a protocol to perfom complex-DNA interaction assay via UV spectroscopy?

10 November 2018 2,812 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

How much costs direct reduction of nitrogen on farmland in terms of lower crop yield?

Dear research fellows, I wonder if someone knows how much direct reduction of nitrogen on farmland costs in terms of lower crop yield expressed as EUR/kg nitrogen (or similar)? I understand that...

15 February 2021 1,323 3 View

What is the paradoxical effect of VINASSE in terms of low pH (<4) and high EC (>50dS/m)? What is its impact on soil properties and crop growth?

What is the paradoxical effect of VINASSE in terms of low pH (50dS/m)? What is its impact on soil properties and crop growth?

15 February 2021 3,142 6 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View