Please suggest whether the method is correct to determine encapsulation efficiency
ex. Suppose you added 100 mg of drug in formulation and total content of your formulation is 1000 mg including drug.
it means 1000 mg powder contain 100 mg drug.
If your yield is 500 mg,
it means theoretically
500 mg powder contain 50 mg drug.
or
50 mg powder contain 5 mg drug which corresponds to 100 % encapsulation
In order to check it practically,
Take 50 mg of formulation, dissolve it in solvent in which your drug and polymer is soluble and measure drug content
if you get 4 mg then
use formula: Practical Drug Content × 100/ Theoretical Drug Content
Encapsulation efficiency= 4 *100/5 = 80 %
Please suggest if it is wrong or right
Hi! Deepak,
Entrapment efficiency gives you an idea about the %drug that is successfully entrapped/adsorbed into nanoparticles. It is calculated as follows:
%EE = [(Drug added - Free "unentrapped drug")/Drug added] *100
Example: If the %EE is 30%, it means that 30% of your drug is entrapped into the nanoparticles.
Loading capacity helps you to deal with nanoparticles after their separation from the medium and to know their drug content. It is calculated using the following equation:
%LC = [Entrapped Drug/nanoparticles weight] * 100
Example: If the loading capacity is 30%, it means that 30% of the nanoparticles weight is composed of the drug! i.e. Each 1 mg nanoparticles contains 0.3 mg drug.
Regards,
Abhishek
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