Please suggest may i use immunoelectrphoresis in helminthes infevtion analysis? if yes then how?

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Can I use reduced graphene oxide/Conducting polymer/Metal oxide based ternary electrode as both anode and cathode in a symmetric super capacitor?

Supercapacitor application

14 October 2020 2,409 6 View

If human elephant conflict is due to faulty forest management practices and regeneration approach?

I have observed from reports in India that more Human Elephant conflict is there in Central and Eastern India compared to Southern India. This raises a question why it is so? one strong reason...

22 September 2020 1,576 3 View

Why do we use HHL as a substrate in studying ACE enzyme inhibition activity assay?

Need to know from the community why we use Hippury-L-histidyl-L-Leucine as a substrate in ACE inhibitory activity

20 September 2020 1,040 5 View

Just noticed with some papers that when we use Box-Behnken design for optimization using RSm, there are three identical runs. Can anyone explain why?

I ran a experiment in design expert and obtained three identical runs which seems weird. Checked few papers and they have three identical runs too and are published. Whats the reason behind this?

14 September 2020 6,091 8 View

Use cases of Internet of Things based solution for COVID-19?

We have identified a use case of IoT (Quarantine people tracking) (paper link)IoT-Q-Band: A low cost internet of things based wearable ban... Is there any other indicative application related...

04 May 2020 8,263 34 View

Regarding thin film coatings ?

I am trying to deposit Titanium monoxide (TiO) on glass using thermal evaporation technique and sometimes i am getting the bluish colour coating ,some mixed with golden shade and sometimes mostly...

04 March 2020 134 4 View

Which method is good for extract preparation?

I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...

09 January 2020 1,257 3 View

Can I store isolated RNA on -20 degree centigrade?

I have isolated RNA from animal tissue. Can I store it on -20 degree centigrade?

04 December 2019 1,888 5 View

How can we establish relation between salt spray test duration & life of component before corrosion starts?

ISO 9227 do not provide any relation to test duration with life

09 October 2019 236 4 View

What are Open Source AI tools that could be used for Automatic Extraction of Exposure data from high resolution satellite imegeries (<0.5 m) ?

Open source AI tools could be great help.

05 February 2019 7,172 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View