Please suggest may i use immunoelectrphoresis in helminthes infevtion analysis? if yes then how?

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Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

Can a Two-Lens System Improve Collimation of a Diode Laser for Scattering Experiments?

Hello Optics community, I'm currently setting up a scattering experiment using a Class 4 blue diode laser with a 10 CW power rating. The challenge is in achieving a tightly collimated beam around...

15 April 2024 986 0 View

Error of Reshape the data in Pythonplot code?

I am trying to plot using the attached file data file, "wan_band.dat". In the past, I also found the same error. But somehow it was resolved automatically. Again, I stuck with it. I am not able to...

09 April 2024 4,738 1 View

Why negative dielectric loss is coming at lower frequency range i.e from 4 to ~100 Hz?

I have performed the temperature dependent dielectric measurements;with proper checking of all electrical connections of electrodes, sensors,heater etc.as well as their proper resistance of...

07 April 2024 7,625 1 View

How substrates can change the mophology of grown metal oxide film?

I'm currently engaged in research focused on the expansion of transition metal oxide thin films. Our approach involves depositing transition metal films on two distinct substrates using either...

02 April 2024 5,783 4 View

Why Raman spectra changes when different laser sources were used for the same sample?

When I was doing Raman spectroscopy, I observed that for the same sample (thin film), using two different laser sources gave different Raman spectra. We know Raman Shift is materially dependent...

02 March 2024 7,770 4 View

What are the diffrent nonpharmacological treatments mentioned in Ayurveda being in practices ?

List of Nonpharmacological treatments modules cost-effectiveness practices PMC publications

06 February 2024 9,814 2 View

HEK293T cells electroporation?

If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...

28 January 2024 8,008 0 View

Is blood isolated monocyte can be kept in incubator in which cancer cell lines are growing?

Dear researchers kindly let me know is it will be feasible to culture blood cells in animal cell culture lab and put the isolated cells to grow in cell culture medium in the same CO2 incubator in...

06 January 2024 1,189 5 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Can anyone help in getting software related to gel scoring of silver stained PAGE for diversity study?

I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...

12 July 2024 7,145 2 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View