I am working on murine model for ischemia reperfusion induced acute kidney injury. I am pretty confused about the type of treatment regimen I should follow- ie whether i should give pre treatment or post treatment to the animals (Wistar rats). Please suggest me the correct treatment plan.
Hi Anuradha,
There are many questions you need to answer before you make this decision. What is your treatment: how do you think it works, how long before it starts to work, what is the half life of the treatment in vivo? Also consider clinical applications: ie. is this a treatment for trauma where in real life, you would not be able to pretreat, or transplantation or some other condition where you could? Finally, look through the literature and see what other people did, especially for your or similar treatments. This will help you decide on a treatment plan. Good luck!
Dear Anuradha,
See some published papers on it, I guess pre-perfusion should be the approach keeping in mind the ischemic tolerance of the nephrons in mind.
Best,
Mannan
Dear Anuradha,
it depends what kind of treatment (chemical/drug) you intend to use: there are hundreds if not thousands of compounds shown to reduce renal IR. The type of chemical dictates the route and timing of administration as well as the dosage. Do not forget to remove the other (non-ischemic) kidney if you want to use creatinin as a marker of injury/recovery.
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