Ashok Kumar

2 Questions 3 Answers 0 Followers

Questions related from Ashok Kumar

What could be probable problem in induction of Protein expression in pET-28a-c(+) vector ?

I ligated ORF of size 1.2 Kb in pET-28a-c(+) vector and cloned in DH5 alpha strain of E.Coli. Restriction release of 1.2 Kb was observed. later i transformed it in Expression strain BL-21 and...

12 December 2017 6,429 2 View

Can anyone help me in troubleshooting of Total RNA Isolation Using Trizol method from wheat leaf (Grain filling stage). ?

I am using freshly puchased ambion trizol reagent. i tried using the protocol provided but not getting intact bands. i thought problem can be due to other reagent and changed chloroform and...

05 May 2016 1,415 8 View