Sudhakar Pagal

4 Questions 5 Answers 0 Followers

Questions related from Sudhakar Pagal

Please suggest a non-red and non-green fluorescent bacterial viability stain?

Hello, My E. coli cells express both green fluorescent protein as well as mCherry. So I need a fluorescent stain of color other than green and red fluorescence to enumerate their viability. Please...

20 March 2024 4,325 3 View

How shall I calculate false discovery rate if I have transcriptome data from more than two categories?

I have three different groups, the aim is to find common genes that are highly expressed in all three groups, as compared to their respective internal housekeeping gene. Thus, I have no control...

22 September 2019 541 1 View

How shall I pull out the best hit matches from the results of a "blastall" local BLAST+ search. I need to build ortholog table of bacterial genomes?

I have 100s of bacterial genome sequences that I have to use to construct an ortholog table by bidirectional all-vs-all BLAST+ search among the protein products. I have already performed a...

13 August 2018 2,760 5 View

Will just amplifying the SacB gene from Bacillus subtilis gDNA and cloning into general vectors like pUC18/19 or pJET will make the vector suicidal?

I am working on knocking out a gene from Acinetobacter baumannii (gram -ve) and need a counter-selectable lethal gene like sacB, but do not have any specialized vectors for that. I am using...

21 June 2016 1,120 7 View