Please provide me with the germination procedure for kydia calycina plants seeds ?

More Yashrajsinh Rathod's questions See All

How are the present IBS-D medicine effective?

The causes of the medicine? how effective it is on humans? how it is made?(i.e how is the medicine made? what are the components used to make it?) total info about its

10 May 2023 4,655 0 View

Permission for Hospital Anxiety and Depression Scale ?

Kindly share the knowledge on how to get permission to translate the Hospital Anxiety and Depression Scale into vernacular language or to get the contact details of the author of the HADS scale.

24 February 2023 9,106 3 View

How to reduce plant mortality rate during second and third transplanting in the nursery stage for casuarina, Haldu?

Sandbed (Mother bed) --> Soil and sand bed --> Polythene bag

26 May 2021 1,199 1 View

For esterification reaction between acrylic resin, acid value=60 and epoxy resin, EEW=880 in presence of DMAE and final acid value=30 wt ratio willbe?

How to calculate weight ratios to carry out esterification reaction between a carboxylic acrylic polymer having Mn=2781 and acid value= 60 and epoxy resin having EEW=880 and Mn=1760 so that the...

09 April 2021 4,199 2 View

How to get an Asymptote from multiple coordinates obtained from Machine Learning ?

I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...

11 October 2019 1,168 2 View

Additive manufacturing by welding, anyone interested in collaboration?

Hi, I would like to lead my research in additive manufacturing by welding and joining.....is there any researcher interested on intellectual ground to collaborate for the research?

23 September 2019 1,372 27 View

Regarding MQTT over TCP?

Hello, I knew that MQTT works on TCP/IP network. But MQTT is application layer IoT protocol. Whether it used all the functionalities of TCP or only some functionalities? If I want to verify...

25 August 2019 6,669 3 View

Salt soluble Proteins ?

Please suggest standard method for determining salt soluble nitrogen and its importance in fishes

22 August 2019 8,154 3 View

Fusion protein won't cut with TEV protease? Any ideas how to obtain higher efficiency in TEV cleavage?

My fusion protein has MBP and small peptide (~5kDa in monomeric form and 25kDa in pentameric form). In between my fusion protein, I have cleavage sequence for Factor Xa protease and TEV protease....

30 July 2019 2,772 7 View

Nominal insolation of laser for PV cell's circuit simulation?

I am working on PV cell's circuit simulation. In the case of solar light we normally use 1000 W/m2 as a nominal insolation but In my case i am using laser diode so what would be the nominal...

11 June 2019 645 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View