Dissolution study on nano co-crystals ?

Hi, SO I prepared nano co-crystals and I have done a dissolution study on them, the thing is I'm getting 130% of my co crystals at the end of dissolution which doesn't make sense !! I have...

26 November 2020 8,816 4 View

Why we can't apply energy or forcefully dissolve lipids when preparing liposomes by thin-film rehydration?

I have read that we can't apply energy or forcefully dissolve lipids when preparing liposomes by thin-film rehydration? I can't remember why or find the source of information, any help?

11 October 2020 3,950 3 View

Difference between polymeric nanoparticles and Liposomes?

What is the difference between PLGA PEG nanoparticles and Liposomes? any recommended papers or reviews that explain the pros and cons of each formulation ??

06 September 2020 6,341 3 View

How to identify the appropriate sites and design a passive seismic survey in order to support the site characterisation?

In the case of overpopulated cities, how I can select the appropriate sites to do some field measurements "Passive Seismic Surveys"? How about the design of the survey in order to study the soil...

04 August 2020 5,253 4 View

How to include or insert the value of LSD (limit of standard deviation ) in an excel graph ?

Please, can a colleges tell me how to include or insert the value of LSD (limit of standard deviation ) of the statistical analysis in an excel graph both (column and line graph)?

04 June 2020 3,835 3 View

Are there any theory/ framework/ model for online teaching?

Are there any model or theory concerning online teaching/learning esecially in EFL context. Are there any model also for emergency remote teaching ?

01 June 2020 3,687 16 View

How can we write research about using technology in teaching while we don't have even stable electricity and internet connection?

It is important for researhers to write about new and up to date topics for example using technology in teaching. In Yemen such topic is difficult to be really tackled because even electricity and...

02 May 2020 2,501 7 View

No title

I need help regarding the solubility of polymeric metal complexes and how to prepare their homogeneous layer on ITO or FTO substrate. i have to prepare working electrode for CO2 reduction

23 June 2018 7,617 2 View

Can I design a survey asking the sample about a technology that they might never actually used?

16 June 2018 5,923 2 View

Can i Investigate acceptance of an idea qualitatively ?

I am embarking on a qualitative research assignment, i want to investigate the acceptance of qualitative and quantitative researchers' on the use of Big Data analysis tools in their research. My...

17 December 2017 4,584 10 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View