Please, i need a detailed information on how to obtain pure culture of Enterococci from urine samples. I intend to use bile esculin agar but i also want to prevent the growth of Enterobacteriaceae. Thank you for your prompt response.
If you use BEA it mostly inhibits other gram-positive bacteria and gram-negative bacteria due to the presence of bile salts(oxygall) and azide, except Group-D Streptococci and Enterococci. Also, It contains the nutrient ferric citrate and Esculin helps to differentiate upon hydrolyzation.