Dear Rume Eni,

Try precipitating saliva proteins using equal volume of organic solvent such as methanol or acetonitrile. Then, vortexing, then centrifugation.

Thanks, can I do this for sugar analysis?

Which sugars?

Glucose and fructose

Yes. But it means you have to use an Aminex (or equivalent) column and an ELSD detector since you are using ACN.

Try doing a little online research.

Article A surrogate method for comparison analysis of salivary conce...