I made fermented milk from mare milk with single probiotic bacteria (Bifidobacterium longum), but need 12 hours to reach 0.3% acidity. The culture cultivated as 10% v/v.
I can think of 2 main reasons why your Bifidobacterium didn't work that much.
Fisrt of all, to my knowledge, (most) Bifidobacteria are strictly anaerobic organisms so that, as a general rule, when you culture them you should avoid even the slightest oxygen concentration...
Then, mare milk is very high in lysozyme (and other antibacterial proteins) compared to bovine milk (and ruminants' milk in general), and this can inhibit Bifidobacteria as well.
Did you try to ferment cow milk with your B. longum strain? Did it work? Usually Bifidobacteria are not as high acid producers as other bacteria (Streptococcus thermophilus, for instance). Also, one other reason I can think about is where does your B. longum strain come from? Milk? Intestine? of what species? Shifting from one medium to another (in terms of oligosaccharides, micronutrients, pH, growth factors and so on), is something that requires time and might not be possible at all...
Good luck for your experiment, I'd be glad to taste your fermented mare milk by the way!
Hello,
it is not related to the mare milk. i think it is related to the strain, may be this strain needs specific minerals or nutrients. check the nutrition needs of this strain.
Please, try to use DVS (direct vat set) starters under normal incubation conditions.
Bifidobaterium strains are generally slower growers and highly anaerobic. The reason is the absence of true yogurt cultures (S. thermophilus and L. bulgaricus), which acidify milk faster because of their symbiotic nature.
You can try inoculating milk at higher concentrations, and try different fermentation temperatures (I would suggest fermenting at 40-42C would work best). Or supplement your milk some antioxidant (such as cysteine) because they can reduce the redox potential of milk and allow faster growth of anaerobic bacteria.
You can compare the growth of your starter in cow and mare milk . if the growth is still slow as you stated , I think that the problem in your starter culture . I think that the lyophilized starter culture is suitable for research work . good luck
Mr Bianchii, c'est sans dout que la presence de lysozyme peuvent inhiber la croissance des bifidobacteries. For others, I found that Bifidobacteries produce only low to mild acid. Despite it, I believe that mare's milk contain oligossacharides which is usefull and act as growth promotters for this bifidobactery. In other research I found it is capable to grow well in media containing stachyose or raffinose.
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