I extracted humic matter using 200g of soil from 1M NaOH and hydrolysed with 1M HCl and then dialysed and refrigerate to purify the samples before NMR analysis.
Mr. Osim,
Generally, there is a question what you expect to obtain as NMR signals, studying humics? You need, in fact, UPLC-MS/MS.
Mr. Osim,
I am sending you a link (below) to a paper on IR-analysis of humics. There are shown the spectra of analytes and a standard (Fig. 3, attachment). The bands within 3020-2800 cm-1 belong to CH3,CH2,CH groups, which are intensive in the spectra. The modes within 1250-1000 cm-1 (fingerprint region) are of C-C, C-O, C-N moleculat motions. They are also strong intensive in the shown spetra, meaning that the number of these functional groups is large.
In this context in your NMR-spectra absent a large set signals for Cs. But when you are OK with your data....
https://www.jstage.jst.go.jp/article/geochemj1966/38/3/38_3_255/_pdf
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