I require help to perform peptide mass-fingerprinting from 50 protein spots (excised from 2d gel and trypsinised thereafter)
1. I have MALDI-TOF/ MS data for secretome proteins from B. anthracis and B. cereus
2. I cannot use MASCOT fingerprint search as it DO NOT have B. anthracisproteome databse
3. i want either licensed version of MASCOT software (I don't have funds to buy the same)
4. I have to create a custom database of B. anthracis and B. cereus proteomes
5. I have to use my data against this custom database to perform peptide mass fingerprinting to identify unknown proteins.
6. please help