Hi Adil,

Do you have any other details of this disease? Such as disease symptom on plant or pure culture of the fungus. Also, are the green cells plant cells or that is the spores you trying to identify?

Cheers,

Rice, Tao-Ho Chang

Hi Adil

I agree with Dr.Tao-Ho, you must give the details about the disease a weed plant

Sincerely

Yes. The big green cells are the spores I want to identify. This picture was taken on 40x magnification of a normal compound microscope. I have uploaded a picture of the disease symptoms. Just the general blighting on leaf tips

The cells look like as the Euglena and similar organisms with plastids, not as fungal spores. See the irregular shape.

Base on the symptom you post Adil. It looks more like a bacterial disease rather than fungus infection. And I agree with Dr. Alena, these green may not be the spores of fungus. In order to identify the pathogen, I suggest you first surface sterilised the leaf and cut leaf in small pieces (with half health and disease part). Then grow them on PDA and water agar. If they are not biotroph, then they will grow on both PDA and WA. Then you could examine the spore or mycellium.

Based on the symptoms, I don't think this is a foliar disease. It looks like stress, which means the problem is lower down in the plant, in the stem, the roots or the soil. Could there be salt stress (including from added fertilizer)? Drought stress? Nematode infestations or root disease? If no problems in the roots or soil, check the xylem in the stems.

It,s seems to bacterial disease and may associat with it in a damp place.

Maybe, or so wet your plants have root rot.

This pic is not shown like fungal spores. Stain with lactophenol cotton blue.

Actually, I don't think they are fungus spores at all. They were green and large, and the shape of leaf parenchyma cells, so maybe that's what they are?

I agree with Deborah, the elements clearly contain chloroplasts and are plant parenchymatous cells

Alfredo