How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Any recommendations for open-source survey form builder, which has option for semantic differential scale questions apart from Likert scale?

Asking this as Google forms doesn't allow multiple items in a semantic differential format.

04 February 2021 5,869 4 View

How can I implement a Generalized Estimating Equation method on spatially autocorrelated time-series data?

I used Generalized Estimating Equations (geepack in R) on my regularly monitored (twice a year) plant abundance data in grid cells to evaluate their trends of density. Since these are time-series...

26 January 2021 3,833 1 View

How to prove possibility/impossibility to analytically solve the functional equation f(a ln x^2)/f(x)=x/2a; and how to find out the solution?

This is my very first encounter with functional equation of this kind, and methods of series solution and differential equations are of not much help. The solution to this problem, or at least the...

25 January 2021 6,053 5 View

How to find nature of extremum of a functional? what are the mathematical prerequisites to understand it?

For a function, usually sign of second derivative (and if it is zero, even/odd index of higher order derivative whose numerical value is zero) is enough to detect whether the extreme point is...

25 January 2021 7,703 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Do you have an example of an algebraic Hamiltonian vector field which possess a finite number of periodic orbits?

Is there a polynomial Hamiltonian vector field with a finite number of periodic orbits? Please see this MO question:...

21 January 2021 1,673 4 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View