Please give a Natural deep eutectic solvents (NADES) . methodology for the extraction of flavanoides?

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How the slurry is preparing for cathodes in batteries?

13 June 2024 4,314 3 View

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world ?

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.

09 June 2024 1,805 4 View

How can analyses a split plot design by SPSS?

02 June 2024 5,946 2 View

What do you mean by meadow plant?

01 June 2024 7,248 2 View

What is the actual difference between Competency-Based Education (CBE) and Outcome-Based Education (OBE)?

In any professional education students' Competency is Evaluated and used as the measurement of Outcome of the teaching-learning process. Then how these two are differentiated in curriculum...

22 May 2024 8,488 25 View

Can I purify mononucleosome from T-cell using a kit followed by SEC superdex 200 10/300 column chromatography for CryoEM ?

I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...

19 May 2024 7,170 1 View

How can I prepare a loading dye that also act as a stopping buffer for an enzymatically degraded DNA, for analysis on a sequencing gel?

I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...

17 April 2024 8,025 6 View

How perform subpixel level measurement of deformation using image processing?

Hi, I am working on subpixel level measurement of deformation using FPGA with the help of image processing technique. Can anybody suggest me the steps to be followed? As per citation pixel level...

14 March 2024 4,078 0 View

What is the performance parameter for imblance dataset ?

I am curr research at phising detection Using URL . Using logistic Regression model . I have data set 1:10 ratio 20k legitimate and 2 k phishing .

10 March 2024 9,531 2 View

Why are some of the Nickel-based Superalloys used in the as-cast condition?

21 February 2024 3,483 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View