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Secondary Structure of a protein is a parameter of its compactness. For example, if by any perturbation, helicity decreases, size (volume) of the protein increases. Except secondary structure,...
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Two most important structural parameter of a molecule is size and shape. While there is many spectroscopic (like DLS, neutron scattering, fcs) and microscopic (SEM,tem, AFM, etc); for shape...
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In general biophysical experiments are carried out maintaining pH of around 7. For this purpose one can use various buffer systems like phosphate buffer, citrate buffer, tris buffer, bicarbonate...
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How to calculate the excluded volume of a Crowder, say dextran-40? If it is 4×volume of the molecule. If we calculate for 1 ml , then at a moderate concentration, the value exceeds 1ml! How? The...
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Intermediates are very important and often found in protein unfolding or foldingbpath. Sometimes, the intermediates are characterized as molten globule states (native like secondary structure and...
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Partition coefficient of a solvent1/ water system might be defined as the ratio of a chemical's concentration in the solvent1 phase to its concentration in the aqueous phase. Solvent 1 is...
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