Please can someone suggest me a methodology for protoplast isolation from pineapple leaves?

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David Roger

Hi,

Have you controlled temperature and stiring speed during digestion step ?

These parameters are very important for the quality and the yield of protoplasts.

I hope it could help you,

David

S. V. G. Nirosha Priyadarshani

Thank you very much the suggestions. I will follow these recommendations in next time.