How to create Online AHP Survey?

Do you know any web tool that I can use to create AHP Survey? I have it as word document and people are not interested to participate is the length is 17 pages. I hope with online survey it will...

21 March 2020 249 2 View

How to praper graphitic carbon nitride from urea ?

I want to know How to praper graphitic carbon nitride from urea ? I need a proven method because I tried several methods that didn't work

27 October 2019 7,236 3 View

I am looking for clay that aggregates and settles slowly in water. Which one is better for aggregation Kaolinite or Montmorillonite ?

I am interested in oil-particle aggregation in fresh water. I have used Kaolinite with DI water and tap water and mixed them in jar test for approximately 30 mins , but i did not notice any...

19 May 2019 646 3 View

What is the difference between nonionic and ionic surfactant in formation of emulsion microcapsules?

I made two experiments to form an emulsion from microcapsules and used two different kinds of surfactant. In the first experiment I used sodium dodecyl sulphate (SDS), it is a anionic surfactant,...

01 January 1970 692 3 View

Does surfactant give me an organic phase?

Can any one tell me please if solution consists of PVA and little ammount of surfactant like SPAN 80 in distilled water can be considered an organic phase? and why? an article I read called this...

01 January 1970 1,192 6 View

Is an instrument can assess thenutrition in children with cebral palcy?

I'm interested in this research - could you provide the full-text for it? or any kind of instrument of assessing nutrition in children with cerebral palsy - Weaam N Alobaidi

01 January 1970 4,842 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View