I have to construct a Meloxicam calibration curve for Rat plasma spiked drug samples in 100ml solution for getting 1000 µg/ml first stock solution from that I will prepare different serial dilution, for getting 1000 µg/ml plasma spiked drug samples first stock solution, I am adding 100mg drug into 99ml solution and then adding 1ml of extracted plasma sample, am I going to correct way? and how i will determine Limit of detection (LOD) and limit of quantification(LOQ )

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