Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How much Morphine Standard is dire need for determination and extraction of Morphine in HPLC technique?

I want to purchase some Morphine Standard for HPLC to detect Morphine rate in Papaver somniferum L. how much is need in every stage or phase?

08 January 2021 1,675 3 View

Hi dear fellow researchers and senior scientists ,i need validate method for Safranin-O-fast green images intensity quantification?

Dear fellows i have images of meniscus sections stained with Safranin-O-fast green. I need to quantify the images for Safranin-O-fast green intensity and any other parameter if...

30 December 2020 1,931 3 View

1.How can put nodes on equal distance in WSN? 2.How calculate retransmission of each node in WSN? 3.How to create buffer for each node?

I am using matlab simulator and doing paper selfish node detection based on average retransmission number. Some factors determine selfish node in wsn -If energy is not sufficient to forward...

28 December 2020 7,294 2 View

What is the blank for catalase activity measurement by spectrophotometer?

I extracted the enzymes of leaves with a potassium phosphate buffer containing PVP and EDTA for catalase activity measurement. The reaction mixture is 3 ml (2.95 ml phosphate buffer+ 0.05 ml...

26 December 2020 606 3 View

How to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS?

Dear researchers, how to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS? How does the abaqus distinguish between the 1d uniaxial stress and strain analysis? Thanks in advance.

20 December 2020 370 2 View

Can you accept for my invitation to join the JAC-ECC Conference, I enclose for you the detailed program?

can you accept for my invitation to join the JAC-ECC Conference, I enclose for you the detailed program?

07 December 2020 8,576 7 View

How to determine/select the indices (underlying weather index) of weather-based insurance?

It should not be confused with strike and limit thresholds. In this case, I am emphasizing the basis of the index, such as: -Cumulative, accumulated, deficit and excess rainfall values. -Average,...

29 November 2020 3,084 3 View

Can anyone help with SDS-PAGE problems?

i'm working on protein evaluation in flour of wheat and others legumes.....I'm using SDS-PAGE. However those days (about two weeks ago) i foudd nothing in the gels (no bands), i dont know what's...

11 November 2020 2,330 3 View

Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,095 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View