Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field conditions (no refrigeration for >1 week)?
According to the manufacture instructions, thin samples (
I have used RNA later for RT PCR, I stored my tissue for couple of weeks in -20 in RNA later
Yes, I always kept RNA samples at -20 degree C. Probably it will work for a long time.
My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...
10 December 2020 885 3 View
My antibody didn't work at all. I have mouse brains, 30 um cryosectioned, and tried different concentrations of tween, as well as both formamide and SSC treatment to no avail.
18 December 2016 6,751 3 View
Collected September 2, 2016 in Donetsk, Ukraine
10 September 2016 930 17 View
I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...
01 February 2016 2,766 17 View
27 October 2015 7,879 3 View
I am looking for suggestions from experts-what they suggest and students-what worked for you?
05 June 2014 3,721 3 View
I'd like to calculate relative energies for some molecules with taking a solvent into account. Reaction takes place in sulfuric acid, which is not included in the list of defined solvents in...
15 October 2013 7,150 7 View
Dear colleagues, We are going to stimulate human B lymphocytes isolated from PBMCs from blood of healthy subjects (negative selection with MyltenilBiotech magnetic beads). It should be a control...
24 May 2012 2,800 29 View
I am working on my thesis and I have a survey that has true/false and multiple choice questions. From what I understand, KR20 is used for dichotomous variables to estimate reliability coefficient....
01 January 1970 789 3 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View