Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field conditions (no refrigeration for >1 week)?
According to the manufacture instructions, thin samples (
I have used RNA later for RT PCR, I stored my tissue for couple of weeks in -20 in RNA later
Yes, I always kept RNA samples at -20 degree C. Probably it will work for a long time.
I am trying to do the affinity purification of MS2-tagged mRNA by capturing it on GST-MCP (MS2 coat protein) immobilized on glutathione beads. I used two approaches: transfecting the construct...
12 June 2024 504 0 View
We cultivate OKT8 hybridoma cells in complete medium with FBS but FBS complicates downstream processing and final product purification. We are going to use HybridomaPlus, Protein-Free Hybridoma...
01 February 2024 9,799 0 View
Share your experience in restoration, restoration of ecosystems, special interest in high mountain systems. Particular interest in the work, methods of implementation and the results obtained.
29 January 2023 8,084 6 View
Botrytis cinerea infection leads to the formation of necrotrophic lesions on plant leaves. In some cases (infection with several B. cinerea strains), these lesions are surrounded by the area of...
22 June 2021 5,782 10 View
My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...
10 December 2020 1,129 3 View
To increase the effectiveness of the activity of control and accounting bodies, it is necessary to build a model for assessing the effectiveness of the bodies of external municipal financial...
16 March 2020 3,085 3 View
I am currently studying the problem with the quality of distilled glycerin. When stored, it acquires a pink tint (see photo). We analyzed glycerol on a gas chromatography mass spectrometer. In the...
01 April 2019 4,463 8 View
Until now, I was relying on KOBAS ( KEGG Orthology Based Annotation System), which was quite user-friendly and provided p-values for distinct pathways. However, it seems to be no longer available....
20 February 2018 7,839 1 View
I am going to submit and publish my scientific paper. The study area is multimodal pedagogy, reading strategies and comprehension. Can you send web page of the Call for papers?
28 January 2018 5,605 0 View
Dear Colleagues, What do you think about what kind of the methods of forecasting of the economic indicators of an enterprise's activity are the best in variable environment condition? I'm...
14 December 2017 7,572 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View