Hi RG,
I am trying to do ploidy analysis of tobacco leaf by Flow. I made single cell suspensions using the protocol mentioned below
Maceration of young tobacco leaves in MgSO4 buffer containing DTT, Triton X-100 and PI > Filtered through cheese cloth > Nylon Mesh (40um)> Centrifuged at 7000 rpm for a minute> pellet collected> incubated with RNAse and PI and acquired in LSR II (10000 events). CEN used as internal controls. While analyzing the plots I see two distinct population with below my 2n CEN standard. Can any one please explain what could this population be. I have attached the PDF. Thank Heaps for all your comments.
Regards
Abhi
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining
Similar questions and discussions