How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

Does anyone have the Grass Instruments/AstroMed S88X Stimulator Manual?

Hello Everyone, Does anyone by chance have a Grass Instruments/Astro Med S88X stimulator manual? (http://www.neurolabparts.com/stimulators/99-grass-astro-med-stimulators-model-s88x.html). I...

17 December 2019 1,447 1 View

Is it mandatory to detect target protein and control (actin or tubulin) in the same membrane?

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30 May 2019 10,014 2 View

Can anybodu suggest a good journal to publish the work on "STEEL TRUSS OPTIMIZATION USING GENETIC ALGORITHMS AND 2D TRUSS ANALYSIS PROGRAM IN MATLAB"?

We have done a work on "STEEL TRUSS OPTIMIZATION USING GENETIC ALGORITHMS AND 2D TRUSS ANALYSIS PROGRAM IN MATLAB". We would like to publish it. So we need good journal to publish the work.

15 March 2018 6,442 2 View

Can anybody explain how to find the permeability of pervious concrete experimentally?

We are working on a project for studying the characteristics of pervious concrete. We are planning for fabrication of a model for finding the permeability of pervious concrete.

13 November 2017 3,567 7 View

Availability of Somatic Mutation Gene Panel in Breast Cancer?

Is there any somatic mutation gene panel for detecting breast cancer? I already know there are Germline mutation gene panel for Breast Cancer

09 July 2017 5,361 1 View

Can anyone help with Ansys 14.1 transient full mode analysis?

06 July 2015 9,580 3 View

Why core shell quantum dots is not dissolving in water or ethanol?

17 February 2015 4,963 3 View

What is the role of copper in anaerobic metabolism?

I want to know the role of copper in anaerobic metabolism and also I like to know the interaction between the nanoparticle and bacteria and how nanoparticle inhibit / enhance bacterial growth.

14 May 2014 9,429 2 View

Can anyone help with this Gaussian error message- "write error in NtrEx1"?

An error message " write error in NtrEx1" is showing when I was doing optimization and frequency calculation in Gaussian 09. Can anyone help me?

04 May 2014 8,930 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View