How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View

Why does using lens (achromatic doublet lens) for imaging detection onto camera affect magnification in Interferometric Scattering (iSCAT) microscopy?

Hello every scientific explorers!! Recently, I have been interested in Interferometric Scattering (iSCAT) microscopy, the scattering-based imaging microscopy. One of the problems I am facing is...

27 December 2020 8,092 2 View

What were the impacts of liberalization and globalization on Russia in the decade following the collapse of the Soviet Union?

I am writing an essay that will examine the negative impacts of globalization by criticizing the neoliberal paradigm through the lens of Russia during the 1990s.

19 November 2020 5,122 4 View

How to measure the point spread function of confocal microscopy using 405 or 440 laser lines?

100- 200 nm fluorescent beads are available from suppliers but only excited at UV (360 nm) or green and red range (i.e., >488 nm). Also. If the objective lens to be evaluated have (NA

18 November 2020 2,096 3 View

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17 November 2020 6,197 5 View

How best to Collimate an LED to feed into a series of fibers?

I am currently using a simple 8mm focal length lens from Carlco to focus my light from a single small LED into a bundle of fibers that have a total diameter of 9mm. The problem I am having is...

11 November 2020 5,332 5 View

Can you suggest a sub-problem and possible objectives regarding to this thesis/research study?

03 November 2020 8,155 3 View

I am working on Lensless Microscopy. Can anybody tell me how to get spectrum of a sample in Fresnel region without using any lens??

I want to get the spectrum of an object illuminated by an incoherent source at the CMOS sensor without using any lens.

01 November 2020 225 2 View

How to measure the focusing efficiency of Fresnel zone plate (FWHM is in micrometer scale) ?

We want to measure the focusing efficiency of the Fresnel zone plate with a very small FWHM. "The focusing efficiency is defined as the ratio of the total power in a circular aperture with a...

01 November 2020 1,252 7 View

Image database of vegetation quadrats?

I am trying to develop a web teaching module (thanks to COVID) on plant community ecology. I am searching some database of photographs from plant quadrats to let students experience the whole...

14 October 2020 8,156 1 View