Hi all,

I am currently searching for an antibody against phosphorylated ABCA1 for western blot. I have a Novus anti-ABCA1 antibody (NB400-105) that is working quite well, but cant seem to find a good one for the phosphorylated protein.

I found a couple online (http://www.emdmillipore.com/US/en/product/Anti-phospho-ABCA1-%28Ser2054%29%2C-clone-EPR2485%2C-Rabbit-Monoclonal-Antibody,MM_NF-MABS734 and http://biossusa.com/store/bs-12956r-hrp.html), but cant find any papers that used them, so Im not sure if they're any good. And all papers I've seen that looked at phospho-ABCA1 used either 32-P or ABCA1 IP and then phospho-serine to determine levels of phospho-ABCA1. We cant use radioactive labeling in our lab, so I may try the IP protocol, but would prefer to just find an antibody against p-ABCA1.

If anyone knows of a good antibody for this, or a paper that used one, please let me know!

Thank you so much for your help!