Ph indicator at Alkaline lysis during plasmid isolation?

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During luciferase assay my miRNA target luciferase gene's coding sequence?

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Does the pressure(-600 mmHg) affects pH?

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Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Why might my GFP tag be dissociating from my plasmid after transfection?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Ehsan Razeghian

The LyseBlue indicator dye added to some of the buffers is Thymophthalein, pH shift from colorless to blue at pH 9.3

Enes Yağız Akdaş

Thanks for your answer,

I have fix the solution by using phenolred

it is light purple in resuspention buffer and become deep purple in lysis step . During neutralisation it becomes yellow