Hello,
I need to determine the appropriate dose for a peptide which aims to inhibit a protein on tumor cells. But I have no idea about how to justify the animal numbers and how to that!
Does anyone have any suggestion or experience?
Thanks
Hiii Raheleh Tooyserkani dose depends on the potency of your therapeutics if you don't have any idea regarding this you should perform bioassay increasing dose manner up to your desirable activity.
You will have to do some experiments to measure the pharmacokinetics of the peptides in the animals (blood or tissue concentration versus time) as a function of dose. Further dose fractionation studies will be needed to understand the pharmacodynamics of the inhibitor.
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