PCR of a 2.5kb product.

03 March 2013 0 6K Report

I've tried to PCR out a 2.5kb insert from PCMVsport6 vector. I used Taq however everytime at different annealing temps I only get a band at ~1.5kb. Can anyone explain why? Primers seem specific enough: Tm~70degC. I tried temps from 58-70C.

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