Does anyone have advice for adherent cells not attaching to multi-layer flasks when scaling up?

I have been culturing human microvascular endothelial cells (HMEC-1) on my own-made low-serum media. The cells expand fine in wells and flasks T25, T75, and T180 using 0.2 ml/cm2, so the media is...

24 November 2023 1,419 1 View