Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

Looking for a manual about NIRS in cognitive science / neuropsychology, suggestions?

Dear community, i'm looking for a manual about NIRS, functioning, methodology and data analysis. in the field of cognitive science / neuropsychology. I would like to know as much as possible...

30 January 2020 8,861 2 View

Could you recommend a book on White Mice brain or on pathology of white mice (Mus musculus) brain?

The book will be used as a reference.

17 March 2018 739 3 View

Does anyone know a replacement for RNA carrier for Qiamp minelute virus spin kit?

I am intended to sequence (via Illumina) viral nucleic acids, including RNA. I was informed that using RNA carrier is not recommended in this case. However, since my samples will have a low...

29 November 2017 8,244 2 View

LPS dosage with LAL QCL-1000 Chromogenic endpoint Lonza, does anyone with experience?

I'll dosage LPS with LAL QCL-1000 Chromogenic endpoint Lonza, and i have many questions about this. Has anyone used this kit yet? Does anyone know if all materials should be depyrogenised? Can the...

03 April 2017 7,719 1 View

Dear colleagues, Can anybody help me identifying these fish vertebrae?

25 May 2016 2,532 1 View

I am starting a research on schools and clubs offering sports. Anyone know any database on the subject?

07 March 2016 9,875 3 View

How can I do PET Spheres/aggregate for concrete?

My question is about some heat treatment for RE-PET from bottles to obtain particles to use as aggregate in concrete.

19 January 2015 7,616 2 View

Where can I find software for data classification using label propagation?

Dear colleagues, I am doing some research in data classification, and I would like to compare to some classification approaches that uses label propagation (see e.g. Zhu, Ghahramani,...

22 October 2014 3,161 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View