Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

Looking for a manual about NIRS in cognitive science / neuropsychology, suggestions?

Dear community, i'm looking for a manual about NIRS, functioning, methodology and data analysis. in the field of cognitive science / neuropsychology. I would like to know as much as possible...

30 January 2020 8,861 2 View

Is implementation of shared/distributed leadership research overlooking the impact of the central leadership figure?

In examining research on shared/distributed leadership, there is a constant rationale for the need of a qualified central figure to maintain the shared/distributed environment. However, the...

20 October 2018 5,099 5 View

Best way of thermal imaging in vitro or in a well plate?

Hi all, I am considering options for thermal imaging in a well plate. I have seen infrared camera used in vivo but so far no papers in vitro with commercially available resources. Could anyone...

29 July 2018 9,732 3 View

Could you recommend a book on White Mice brain or on pathology of white mice (Mus musculus) brain?

The book will be used as a reference.

17 March 2018 739 3 View

Does anyone know a replacement for RNA carrier for Qiamp minelute virus spin kit?

I am intended to sequence (via Illumina) viral nucleic acids, including RNA. I was informed that using RNA carrier is not recommended in this case. However, since my samples will have a low...

29 November 2017 8,244 2 View

Any way to salvage data from merged colonies in a colony forming assay?

We attempted a series of colony forming assays of cancer cells for irradiation. However, due to some vibration issues from our incubator, the cells settled towards the center of the plate...

27 July 2017 5,593 1 View

LPS dosage with LAL QCL-1000 Chromogenic endpoint Lonza, does anyone with experience?

I'll dosage LPS with LAL QCL-1000 Chromogenic endpoint Lonza, and i have many questions about this. Has anyone used this kit yet? Does anyone know if all materials should be depyrogenised? Can the...

03 April 2017 7,719 1 View

How to dry oleate salts?

22 February 2017 2,997 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View