Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

23 June 2015 3,340 3 View

Can someone give me a suggestion on the cloning procedures?

11 March 2015 1,728 4 View

Does anyone have experience with, or literature on, bone plow or rake phenomenon using locking plate fixation ?

When bone is cut by locking screws. In my experience in metaphyseal bone.

08 February 2015 5,366 2 View

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I have a list of genes annotated with InterProscan. I would like to summarize the results plotting the GO category and look at which major function or biological processes those genes are involved...

12 August 2014 455 2 View

Who knows sites hosting free samples of images with known clusters inside to test clustering machine learning models?

I have several clustering models to be tested and validated on images for which clusters inside are well known.

23 March 2014 7,277 2 View

Could somebody please give me the nucleotide sequence of the TEV protease recognition site?

I would like to use this sequence for TAP of protein complexes.

17 February 2014 7,498 7 View

Which activities should be the most important for academic people?

Normally an academic dedicates do some hours per week to research, some to teach and some to administration of the Department or Faculty. Some hours are also dedicated to external work, as...

01 November 2013 4,783 3 View

Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

I am trying to identify histone locus bodies in a prostate cancer cell line (PC-3)

09 July 2013 280 3 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

20 June 2013 1,849 19 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

05 April 2013 5,771 10 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View