Hi,

I am looking for an optimum overnight-storage condition for my freshly isolated dendritic cells. As it takes almost a whole day to isolate dendritic cells from intestinal lamina propia of mouse, it is difficult to start cell stimulation and straining on the same day of cell isolation. Therefore, I would like to keep the freshly harvested cells overnight and continue staining from the following morning. Does anybody have similar kind of experience? Can I keep the cells in PBS at 4C overnight without disturbing its cytokines expression ability? It is highly appropriated if someone suggests any other suitable way for overnight-storage of dendritic cells to be used for cytokine measurement by CyTOF.

Thank you for your time and help.

Best Regards

Zohorul