Outsourcing of cloning/plasmid preparation for dual luciferase assay in India?

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Does there exist any empirical evidence of government expenditure financed by money collected through lobbying?

Basically, i am trying to understand from the literature whether lobbying can also considered an alternative source of financing fiscal deficit.

26 July 2024 8,692 5 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

RNA isolation from plant roots infected with both bacteria and fungi. How to isolate RNA from all of them simultaneously?

I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...

09 June 2024 9,876 0 View

How can I find capacity of Li-Ion coin cell (CR2023) battery ?

if I want to run my battery cycling test in 1 C rate ,but then I need to know the capacity of my material used in the coin cell (i.e. if my capacity is 20mAh then I will put a constant current of...

05 May 2024 1,364 1 View

Are there any suggestions or insights you can provide to help address this convergence failure in my optimization process?

I'm currently experiencing convergence failure while attempting to optimize a dimeric liquid crystal molecule under the influence of an electric field. The optimization code I'm using is...

10 March 2024 4,737 0 View

Does the dipole moment of a molecule correlate with its hyperpolarizability and polarizability?

implying that a molecule with a lower dipole moment exhibits lower hyperpolarizability and polarizability compared to one with a higher dipole moment?

07 March 2024 572 2 View

What is the script for running protein cluster by using DBSCAN?

Im trying to run dbscan.py by using vmd (dcd and pdb) files but the script is showing error. Its not generating cluster it's only generating noise from the trajectory file. How to solve this issue...

03 March 2024 6,414 0 View

How can I effectively analyze my data using multinomial logistic regression in SPSS?

I am conducting a study that involves analyzing categorical outcome variables with multiple levels and want to employ multinomial logistic regression analysis in SPSS for my data. However, I'm...

29 February 2024 8,533 4 View

Why is my IS high in plasma when measured in GCMS?

Hello everyone, I am currently working on developing a method to quantify butyric acid in plasma on single quad-GCMS using electron impact mode. For this purpose, I am currently following this...

14 February 2024 3,919 0 View

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes?

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?

29 January 2024 3,222 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View