Optogenetics labs in India - can anyone help?

More Manish Joshi's questions See All

I have some queries about basics of neuroscience...?

1. There is much greater concentration of K+ ions inside the cell than outside, why then is the resting membrane potential always negative? 2. If you poison a neuron with a metabolic inhibitor...

09 October 2015 4,314 2 View

How can CoCl2 mimic hypoxia?

Hello, I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

11 December 2014 903 4 View

Why am I getting a nonspecific HIF-1alpha blot?

Hello, I am getting nonspecific bands in HIF-1alpha blot. Can any one suggest why and how I can improve this? I am using the following conditions Cell: HCT116 Gel: 8% Blocking: 5% non fat milk...

10 November 2014 4,956 5 View

How do you induce Hypoxia in cancer cell lines?

Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...

09 October 2014 4,860 7 View

How do you protect proteins from precipitation and degradation?

Hi, I am purifying proteins for crystallization, but sometimes they precipitate and degrade. What are the tips and precautions I should take during purification and storage? Is there any...

09 October 2014 777 14 View

How to identify Drosophila Mutant...?

I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...

08 September 2014 8,253 4 View

How do you check Sensitivity of PCR ?

Hello, I want to know that how to check sensitivity of PCR ?

08 September 2014 313 8 View

Why are tumor suppressor proteins down regulated in cancer cells?

Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...

08 September 2014 4,881 4 View

Can anyone provide me a protocol for Fusarium oxysporum culture?

I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?

02 March 2014 10,128 13 View

Does quantum mechanics fit to explain such brain functions as behaviour, neurodegeneration and consciousness?

Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?

01 February 2014 8,805 46 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

What is the difference between neuropsychology and cognitive neuroscience ?

The main differences

14 July 2024 3,158 4 View

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

06 June 2024 2,234 1 View

Internationally recognised publishing outlets specialised in publishing science-based futuristic papers?

Does anyone use or know of any internationally recognised and reliable publishing outlet (i.e. Journals) that are specialised for submission of "futuristic" articles which are based on the...

28 May 2024 2,946 2 View

How can we effectively model RPE signals at the single-trial level in EEG data?How can researchers disentangle overlapping ERP components effectively?

Dear all, I need your expertise on this matter. I have an article under review and I got some comments that I need your help with: In my research, I investigated the FRN (200_300ms post_feedback...

23 May 2024 4,953 0 View

Can anyone explain why my EGFP expression increased (but Ai9 RFP decreased -like normal) after imaging with a confocal microscope?

I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...

23 May 2024 7,776 2 View

Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

12 May 2024 4,614 3 View

Should a biphasic electrical stimulation pulse on neural networks be positive followed by a negative phase or negative followed by positive phase?

I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...

09 May 2024 7,167 1 View