What do you do when you have tried everything to transform your yeast strain and you see a lot of colonies but most of them are false positives.
Regarding confirmation of transformation, I isolate the gDNA and then perform a PCR but for some reason I do not see any bands even with the positive control. My technique is fine and I tried every possible change with no results. HELP ME!
Katie A S Burnette
If you aren't getting your positive control to work, then the issue is with the PCR. My first guess is that your primers have degraded.
Make a new dilution from the stock tube or order a new tube.
Make sure your controls are working before you try to do any PCR of your samples.
Good luck!
0 votes
0 thanks
- Badges
- Science topic
More Darshi Hemani's questions See All
Similar questions and discussions