Hey all,

Posted this on the imageJ forums, but maybe someone here can help...

I’m trying to analyze some angiogenesis pics, and have finally gotten Angiogenesis Analyzer to finally run, but now am unable to determine the right settings. Every setting I change gives me some version of this pic below:

Output:

https://www.dropbox.com/s/n60a2k4onbvwqxz/Test.PNG?dl=0

Here is the original:

https://www.dropbox.com/s/b6kah2s44f0wrn2/Composite.tif?dl=0

Here are my settings (but I've tried changing a few of the numbers with no luck of making it look better):

https://www.dropbox.com/s/3xpl105xrpavgph/settings.PNG?dl=0

I’ve tried following along with the documentation, but nothing seems to impact the outcome much. can anyone provide some guidance?