Interested into clarifying the estimation parameters of recombinant culture strain of yours using a generic approach? If a culture is fast growing (like E.Coli) then cumulative oxidation capacity helps to identify not only biomass concentration, but also the target protein as in our latest papers
at journal Microbial Cell Factories (Article Generic estimator of biomass concentration for Escherichia c...
) andat journal Entropy ( Article Identification of Functional Bioprocess Model for Recombinan...
)Feel free to share your data to get parameters for your online estimation or suggest your approach which outperforms existing approaches. As an example, Luedeking Piret equation helped us to outperform ANN (artificial neural networks) with less inputs, and less implementation details.
Still do not know how to deal with the estimation of the number of cells in "slow growing" mammalian or stem cells cultures, because gas analyzers miss accuracy at low oxygen consumption rates? How about deriving your own estimation parameters based on the generic models and how about maximizing target protein by systematical control of your cultivation variables like we do.
Or maybe you can suggest your own methodology, which we could benchmark or stress test with data from the past? Feel free to post a paper here, share your experience or directly contact us.
.