Hello, everyone.
I synthesized some carboxyl magnetic beads coupled with NH2-barcode using 0.1 M MES and 0.125 mg/μl EDC, and then connected barcode with d(T)25 tail. The beads were stored the beads in TET buffer (10 mM Tris-HCl, pH 8.0, 10mM EDTA, 0.01% Tween-20) at 4 ℃. After the synthesis, the beads were sampled and tested by a FAM-d(A)25 probe, and they showed bright green light under microscopes.
But five days later I tested them with FAM-d(A)25 probe again, their fluorescence intensity was strongly reduced. Then I tested the beads with FAM-anti-barcode probe and found their fluorescence intensity was also strongly reduced. I have proven there was no contamination of bacteria, and I don't think nuclease could be active in 10 mM EDTA.
Have you ever had a similar problem or could you provide me with any advice?
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