I am not looking for a PCR calculator. There are many PCR calculators that claim to calculate the melting temperature "Tm", but this is false. They calculate polymerase-dependent values of interest for experiments involving polymerases.
Instead, I would like to calculate the melting temperature, as it would be revealed by a melting curve experiment. No polymerase is involved in this experiment, and the value does not depend on polymerase selection. It depends only on sequence and buffer composition.
Do you know of a software that does that well?
The user can select from a variety of Tm calculation techniques using this tool, including those from Santa Lucia 1998, Breslauer, Sugimoto, Howley, and others.
http://resource2.ibab.ac.in/exprimer/
https://www.sciencedirect.com/science/article/abs/pii/S0168165621002777,
It is a nice question to point out. The fundamentals calculation may address the energy content within the oligomer.
