dear friends,
when the analysis of dairy wastewater ( from milk industry) for oil and grease by partition gravimetric method (extraction with n- hexane) according to APHA 5520 B method, how can we extract the fat part of the sample. fat tends to form emulsion and does not extract to hexane. when you add alcohol to break the emulsion, it is with the aqueous layer ( not extract to hexane layer).then the oil and grease value is less than the expected value.
A coagulated chemical such as aluminum or iron salts with industrial waste water, after which the mixture is deposited. Raising the temperature sometimes helps to break down the emulsion, and the amount of sludge produced in this case is large and difficult to dr
Hi,
You can use heat to remove the solvent and the residue is the fat.
by extraction you have to separate the milk fat and potential oil contamination from processing, from milk proteins and potential cleaning agents.
Maybe a drop in pH by acidification can make proteins and anionic surfactants ineffective in stabilizing emulsions.
One can use centrifugation to enhance separation of the hexane. In addition you may also try to add sodium sulfate.
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