Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Does anyone ever solved this following Matlab error : "Error using imaqdevice/start (line 95) pointgrey: Failed to stop the camera acquisition." ?

I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...

22 February 2021 6,386 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Is there any way to possess not water soluble compounds such as apigenin, quercetin, phytosterols etc. in our water based herbal extraction?

Dear Sir or Madam I know that we can obtain these compounds with different solvents. However, i can not use this kind of extract in my product (food supplements or cosmetic). For food supplements...

09 October 2017 9,885 4 View

Problem in docking energy calculation using iGEMDOCK for high molecular weight protein?

I am using iGEMDOCK software to calculate the docking binding energy for my legand compound with protein 2cr0 but it shows error and says that molecular weight is high for this protein is there is...

31 December 2016 1,918 1 View

How we can calculate beta sitosterol content of a sample by GC (after saponification and dervatization)?

In order to measure phytosterol concentration of O/W emulsion, I have used single point internal standard method i.e. calculating the response factor, using areas of known amount of IS and the...

28 October 2016 112 5 View

How we can calculate beta sitosterol content of a sample by GC (after saponification and dervatization)?

In order to measure phytosterol concentration of O/W emulsion, I have used single point internal standard method i.e. calculating the response factor, using areas of known amount of IS and the...

09 October 2016 1,504 6 View

How can I determinate phytosterols amount in a capsule made by ionic gelification? Which is the methodolgy?

07 August 2016 5,788 2 View

Hello dear Scientists. How every one doing?

06 July 2016 4,420 1 View

TMS sterol derivatives are not dissolving in hexane, what could be the possible reasons?

Phytosterols or phytonutrients, chemistry

05 June 2016 8,028 1 View

Dear Scientists. Can any one tell me the specific and most effective method for the extraction and screening of Phytosterol, specially Taraxasterol?

Phytosterols (taraxasterol)

02 March 2016 614 2 View

Is that is possible to call sitgmasterol as a drug candidate for CNS?

04 January 2016 4,623 2 View

Is that is possible to call sitgmasterol as a drug candidate for CNS?

31 December 2015 7,244 2 View