i isolated sterols from plants methanolic extract. when run by TLC , found it is not active under UV and it is sterol by doing acidic methanolic spray with heat treatment 100 c approximately and also chemical test was positive for phytosterol. I would like to know mass of the compound which GC/LC MS Technique is suitable to know mass of the compound. and how should should i know my compound was volatile or nonvolatile.
Yes, it is better to know if the compound is volatile and thermally stable before you can run direct GC-MS . Also, run UV spectra from 200-400 nm to check if it has any absorbance in the range. If non-active you can only use HPLC with an ELSD detector to determine the purity first. There are other ways to find the structure such as NMR.
To know the mass of the compound, you should use gas chromatography-mass spectrometry (GC-MS), which is a technique that combines gas chromatography (GC) and mass spectrometry (MS). GC-MS can separate, identify, and quantify the components of a sample based on their mass-to-charge ratios. GC-MS can be used to study liquid, gaseous, or solid samples.
The sample is vaporized and carried by an inert gas through a column coated with a stationary phase, where the components are separated based on their boiling points and polarities. Then, the components are ionized and fragmented by the mass spectrometer, and the ions are separated and detected based on their mass-to-charge ratios.
The mass spectrum of each component can be used to identify it by comparing it with a library of known spectra. GC-MS is widely used for the analysis of organic compounds, such as hydrocarbons, alcohols, aromatics, pesticides, steroids, fatty acids, and hormones. It can also be used to analyze compounds that are not volatile in their original state, but can be converted to volatile derivatives.
You can roughly understand the molecular weight and stability of the extract, using liquid phase for thermally unstable large molecules and gas phase for thermally stable small molecules.
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