What are some good polymer to use for packaging a biological product ,which can be steril by gamma?

As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...

22 December 2020 8,196 5 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

22 October 2020 9,855 3 View

Which software is better for 3D design of Scaffold Structures for Tissue Engineering?

Now I'm using Sketchup, which is not so appropriate for small objects, and during my web searches, I found some open Source Softwares that specially designed for this purpose (tissue engineering)...

30 September 2020 2,403 4 View

Can themes emerging in findings be different than literature review?

In regards to findings for qualitative research, can the themes emerging be different than what's written in litertature review. They may have been covered by some studies but that is not...

09 September 2020 5,358 16 View

What are the types of optimization problems that can't be tackled by meta-heuristic and heuristic algorithms?

Any decision-making problem when precisely formulated within the framework of mathematics is posed as an optimization problem. There are so many ways, in fact, I think infinitely many ways one can...

06 September 2020 9,415 14 View

Spatial Prediction of Categorical variables with ANN model in R environment?

I would like to predict soil classes with artificial neural network (ANN) in the R environment. I train my model with neuralnet package. When using the predict function, I will only get the...

15 February 2020 4,020 3 View

How do I judge the goodness of my genetic algorithm code?

How to ensure that my ga code is good enough? I guess I should test it on various test functions that are different in structure, that's one obvious thing but, what about the number of chromosomes...

18 October 2019 518 3 View

Is there any GAMS source code for solving multi-objective optimization problems (Aggregate Production Planning)?

I also have question about Energy consumption, is there any way to find each product energy consumption? Is the cost of Energy consumption increases by increasing production (number of products)?

21 September 2019 4,256 1 View

Why some mathematicians are against meta-heuristic and heuristic algorithms?

I have found that some mathematicians disagree with meta-heuristic and heuristic algorithms. However, from a pragmatic point of view such algorithms often can find high-quality solutions (better...

19 August 2019 7,033 97 View

Can any one identify the me the "Nitrate" in this SEM Photograph. The sample is taken from a concrete specimen of a Fertilizer company?

The SEM photograph is attached

08 May 2018 5,104 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View