Number of cells to use for CRISPR KO screening using human GeCKOv2 pooled libary?

08 July 2020 0 9K Report

I'm new to CRISPR screening, please suggest how many cells to use for transduction if I want to use both half-libraries from GeCKOv2 human pooled library (1 vector system) from Addgene!

Thanks,

Ranjitha

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