Should lysis buffer be fully removed or should some be left behind during DNA digestion?

I've been having issue with extracting/precipitating DNA from mouse tail pieces and am unsure if the lysis buffer I use could be the problem. After digesting for 2 hours with lysis buffer and...

04 February 2021 1,364 1 View

How to dissociate and culture retinal cells from mouse eyes?

We have a transgenic mouse line in the laboratory from which we are looking to culture retinal cells from. Our experiments indicate we might be able to produce stable cultures from these cells...

03 January 2021 7,200 6 View

What are the social factors that could affect teacher's behavioral intention to use online learning?

What are the social conditions that may influence one's accessibility to digital content?

30 July 2020 3,539 2 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

Is it necessary to go through an IRB if you want to conduct research independently (no affiliation with a university or organization)?

This question is regarding researchers who are interested in conducting research after grad school but are not affiliated with an organization or university.

23 June 2019 3,779 4 View

Can a PAP pen be used to prevent live cells growing outside a desired border?

I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...

18 March 2019 202 2 View

How do you modify in-text citations on Mendeley so that they are organised chronologically instead of alphabetically?

Hello, I am using Mendeley as my reference manager. I currently have a merged in-text citation: (Atterbury et al., 2007; Ramesh et al., 1999; Smith & Huggins, 2009) I want it to be...

07 March 2019 393 7 View

Where can I find out where to buy the anti-PSA antibody used in the clinic?

I'm testing a new anti-PSA antibody, and I want to compare it to the one used at the moment in the clinic to detect prostate cancer. Does anyone know how to find out where this antibody comes...

21 January 2019 856 1 View

Can anyone identify the species of this mold ?

I'm a food science student that likes to try out with different recipes from time to time. So I have made a really odd wine reduction + plain yogurt panna cotta that taste awfully odd (and super...

31 October 2018 563 4 View

Spatial analysis in R?

27 July 2017 8,562 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View