Hi, Iam using donkey antirabbit secondary antibody at the 700 channel to detect my protein of interest (1;1000 dilution primary ). My problem is that the green channel is showing non specific binding, but the red channel in the same membrane is fine. I used a dilution of 1:15000 and 1:20000, but the results are same. Its a new anitibody and for some time now Iam facing this issue. When I contacted the company they say, they didnt have any issues reported. can anyone let me know how to reduce the background fluorescence. The membrane is immobilon FL as recommended by the company

I have attached the picture. Suggestions are welcome

Jaya

More Jaya Aseervatham's questions See All
Similar questions and discussions